EVALUATION OF THE ANTIBIOFILM EFFECT OF FLUCONAZOLE LOADED PLGA NANOPARTICLES PREPARED USING RHAMNOLIPID

In this study, fluconazole (FLZ) loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) were prepared with two different formulations consisting of polyvinyl alcohol (PVA) and PVA-rhamnolipid (R) in order to improve antibiofilm activity against Candida albicans ATCC 90028. The encapsulation efficiency, drug loading capacity, in-vitro release, characterization these compared. Characterization NPs analyzed by scanning electron microscopy (SEM) Zetasizer. Drug capacity efficiency percentages measured spectrophotometric method. PLGA-NPs spherical shape mean sizes ~300 nm surface charge FLZ PVA PVA-R-PLGA -25,9±1.99, -48,1±2.46, respectively. Sustained release (≥60% after 6 h) obtained PVA-R PLGA-NPs. PVA-FLZ-PLGA PVA-R-FLZ-PLGA 50% 85%, Antibiofilm inhibition are 55% 63%, These results show that the delivery system is a new therapeutic approach can be used infections caused C. albicans.